phosphorylated sites Search Results


h1 phosphorylation  (Santa Cruz Biotechnology)
85
Santa Cruz Biotechnology h1 phosphorylation
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
H1 Phosphorylation, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylation of tyrosine  (Blackwell Science Ltd)
90
Blackwell Science Ltd phosphorylation of tyrosine
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Phosphorylation Of Tyrosine, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal antibodies specific for phosphorylated serine or threonine residues in sq sites representative of atm/atr substrates  (Verlag GmbH)
90
Verlag GmbH polyclonal antibodies specific for phosphorylated serine or threonine residues in sq sites representative of atm/atr substrates
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Polyclonal Antibodies Specific For Phosphorylated Serine Or Threonine Residues In Sq Sites Representative Of Atm/Atr Substrates, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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custom phosphorylation site-specific antibody  (Biomol GmbH)
90
Biomol GmbH custom phosphorylation site-specific antibody
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Custom Phosphorylation Site Specific Antibody, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylation site specific py330 pka-c polyclonal antibody  (YenZym Inc)
90
YenZym Inc phosphorylation site specific py330 pka-c polyclonal antibody
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Phosphorylation Site Specific Py330 Pka C Polyclonal Antibody, supplied by YenZym Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylation motifs  (Kettenbach GmbH)
90
Kettenbach GmbH phosphorylation motifs
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Phosphorylation Motifs, supplied by Kettenbach GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phb2 phosphorylation site-specific mutants (phb2s91a/s91d/s176a/ s176d)  (Cyagen Biosciences)
90
Cyagen Biosciences phb2 phosphorylation site-specific mutants (phb2s91a/s91d/s176a/ s176d)
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Phb2 Phosphorylation Site Specific Mutants (Phb2s91a/S91d/S176a/ S176d), supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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arx ser 266 site-specific phosphorylation antibody  (ABclonal Biotechnology)
90
ABclonal Biotechnology arx ser 266 site-specific phosphorylation antibody
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Arx Ser 266 Site Specific Phosphorylation Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylation sites version 1.3 broad signaling pathway screen  (Kinexus Bioinformatics Corporation)
90
Kinexus Bioinformatics Corporation phosphorylation sites version 1.3 broad signaling pathway screen
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Phosphorylation Sites Version 1.3 Broad Signaling Pathway Screen, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho-site-specific antibodies against rat melanopsin phosphorylated at ser-381  (Pacific Immunology)
90
Pacific Immunology phospho-site-specific antibodies against rat melanopsin phosphorylated at ser-381
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Phospho Site Specific Antibodies Against Rat Melanopsin Phosphorylated At Ser 381, supplied by Pacific Immunology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody that recognizes its hyperphosphorylated form, with a specific site of phosphorylation at serine 409/410  (Agenus Inc)
90
Agenus Inc antibody that recognizes its hyperphosphorylated form, with a specific site of phosphorylation at serine 409/410
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
Antibody That Recognizes Its Hyperphosphorylated Form, With A Specific Site Of Phosphorylation At Serine 409/410, supplied by Agenus Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mass spectrometry (ms) for phosphorylation sites and arh w22x protein sequence analysis  (NextGen Sciences)
90
NextGen Sciences mass spectrometry (ms) for phosphorylation sites and arh w22x protein sequence analysis
FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone <t>H1</t> and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk <t>phosphorylation</t> of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).
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Image Search Results


FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone H1 and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk phosphorylation of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).

Journal: Endocrinology

Article Title: Thyroid hormone-induced cell proliferation in GC cells is mediated by changes in G1 cyclin/cyclin-dependent kinase levels and activity.

doi: 10.1210/endo.140.11.7145

Figure Lengend Snippet: FIG. 2. T3 stimulates cyclin E-associated kinase activity. Cell ex- tracts were prepared at the indicated times after culturing of GC cells in the presence or absence of T3. Cyclin E immunoprecipitates were assayed for kinase activity in the presence of histone H1 and [g-32P]ATP as described in Materials and Methods. Kinase reactions were subjected to SDS-PAGE followed by autoradiography. Results shown are representative of two independent experiments. A, Auto- radiograms of cyclin E-cdk phosphorylation of histone H1. B, Quan- titative analysis of the results shown in (A). The results were quan- titated with a PhosphorImager and values expressed as cyclin E-associated kinase activity of cells cultured in the presence of T3 relative to that of cells cultured in the absence of T3. Kinase activity obtained by performing the experiment in the absence of anti-cyclin E antibodies was substracted from its corresponding experimental group. Each time point represents the kinase activity from four in- dividual dishes. Data are expressed as mean 6 SD n 5 8. Statistical significance between the different groups was shown by ANOVA followed by Fisher’s PLSD (P , 0.05).

Article Snippet: Immunoprecipitation, in vitro kinase assay, and Western blot analysis For immunoprecipitation, either 400 mg (H1 phosphorylation) or 100 mg (GST-Rb phosphorylation) of protein extract were incubated with 20 ml of Protein A/G Plus-Agarose (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) for 1 h at 4 C. The proteins binding nonspecifically to protein A/G Plus-Agarose were pelleted by centrifugation at 4,000 3 g for 5 min and the supernatants incubated for 3 h on ice with 4 mg of anti-cyclin E (sc-481) or anti-cyclin D1 (sc-450) for histone H1 or GST-Rb phosphorylation, respectively.

Techniques: Activity Assay, SDS Page, Autoradiography, Phospho-proteomics, Cell Culture